Research Article

Study of the association of reduced level of adinopectin, vistafin and eslastase with increased risk of diabetes in a group of first degree relatives of middle-aged Pakistani people.

Gul-e-Raana and Rukhshan Khurhsid

Department of Biochemistry, Gujranwala Medical College, Gujranwala and Fatima Jinnah Medical College Lahore Pakistan.

*Corresponding author: rakhshan99@yahoo.com,

Received 27 February, 2013; Accepted 25 March, 2013

Abstract

A cross sectional study was designed to observed that the reduced level of plasma adiponectin and visfatin concentrations may be associated with decreased sensitization of insulin and increased risk of diabetes in first degree relatives of diabetics. We have studied 50 subjects (aged between 26-35 year-old) who had a family history of diabetes during 2010-2011. 20 age matched control subjects without a family history of diabetes were also taken. A questionnaire on demographics, history, clinical examination and laboratory tests was filled out for each patient. Plasma glucose was measured using the GOD-PAP enzymatic method. Level of insulin was measured by ELIZA technique. Raw volume of adinopectin, visgatin and elastase was estimated by 12% SDS polyacrylamide gel electrophoresis. Their BMI and insulin resistance was calculated. Results showed that mean age of first degree relatives was 29.67 year. Their BMI was non significantly greater than their controls. Level of fasting blood sugar, serum insulin and insulin resistance were increased as compared to their controls but significant difference was only observed in case of level of serum insulin. Raw volume of adinopectin, visfatin and elastase was non significantly decreased in FDR as compared to their controls. It is concluded that diabetes screening including estimation of visfatin, adinopectin and elastase is an effective means of detecting unknown cases of diabetes among FDR, improving glycemic control and mitigating CVD risk factors.

Keywords: First degree relatives, diabetes, visfatin, elastase, adinopectin.